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ASCO 2025 | Tissue-free versus tissue-reliant ctDNA testing in solid tumors

John Paul Shen, MD, The University of Texas MD Anderson Cancer Center, Houston, TX, compares recent methylation-based tissue free testing to traditional tissue-dependent circulating tumor (ctDNA) approaches. With the ongoing consensus that sensitivity changes based on metastatic location and somatic mutations of the tumour, further trials are necessary to confirm how this new method will differ in specificity and sensitivity in detecting molecular residual disease in circulating tumour DNA. This interview took place during the 2025 American Society of Clinical Oncology (ASCO) Meeting in Chicago, IL.

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Transcript

We’re still, I think, learning a little bit about specificity and sensitivity, you know, even with the tissue-based assays. We have other work not presented here, but that sensitivity definitely changes depending on metastatic location and sometimes on the somatic mutations of that tumor. We have not tested this new tissue-agnostic methylation-based assay in enough cases head-to-head to really make a comment on the direct comparison, but we think that it is going to be very similar...

We’re still, I think, learning a little bit about specificity and sensitivity, you know, even with the tissue-based assays. We have other work not presented here, but that sensitivity definitely changes depending on metastatic location and sometimes on the somatic mutations of that tumor. We have not tested this new tissue-agnostic methylation-based assay in enough cases head-to-head to really make a comment on the direct comparison, but we think that it is going to be very similar.

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Disclosures

Stock and Other Ownership Interests – Agios; Syndax; Syndax
Consulting or Advisory Role – Engine Biosciences; NaDeNo Nanosciences
Research Funding – BostonGene; Ikena Oncology (Inst)