Atocha Romero, PharmD, PhD, Hospital Universitario Puerta de Hierro, Madrid, Spain, provides an overview of the remaining challenges prior to the implementation of extracellular vesicles-based noninvasive testing, including characterization of the vesicles containing the biomarker of interest and the development of high-throughput technology to capture the vesicles. This interview took place at the IASLC 2022 World Conference on Lung Cancer congress in Vienna, Austria.