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ASCO 2026 | Why RNA sequencing complements DNA testing in precision oncology

Takao Fujisawa, MD, PhD, National Cancer Center Hospital East, Kashiwa, Japan, emphasizes the importance of performing RNA sequencing in conjunction with DNA sequencing and other multi-omics analysis to expand target variable biomarkers. RNA sequencing can detect fusion variants, target RNA expression, and provide valuable information for cancer biology, while DNA sequencing detects key genomic alterations such as short variants, TMB, and MSI. This interview took place during the 2026 American Society of Clinical Oncology (ASCO) Meeting in Chicago, IL.

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Transcript

We medical oncologists should perform the RNA sequencing in parallel with DNA sequencing and other multi-omics analysis because they can expand the target variable biomarkers. And as you know, the DNA sequencing can detect the important genomic alterations like the sort of short variants and some of the important variants like TMB or MSI. And the RNA sequencing can detect the fusion variants and target RNA expression...

We medical oncologists should perform the RNA sequencing in parallel with DNA sequencing and other multi-omics analysis because they can expand the target variable biomarkers. And as you know, the DNA sequencing can detect the important genomic alterations like the sort of short variants and some of the important variants like TMB or MSI. And the RNA sequencing can detect the fusion variants and target RNA expression. And we also perform the AI signatures using the RNA sequencing because the RNA sequencing has a lot of information for the cancer biology. So in conclusion, we think that to perform the comprehensive molecular testing, including the RNA, DNA, and other multi-omics integration can expand the precision oncology.

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